- 询价
- Immunoway
- YM6839R
- 2025年07月10日
- IHC
- 小鼠
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse, Monoclonal/IgG2a, Kappa
- 形态：
液体
- 保存条件：
2-8℃
- 克隆性：
单克隆
- 适应物种：
Human
- 保质期：
一年
- 级别：
病理级
- 库存：
100
- 供应商：
ImmunoWay Biotechnology Company
- 宿主：
小鼠
- 应用范围：
IHC
- 规格：
3ml/6ml/10ml
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
pellets in 10 ml of 100 mM Tris buffer or Binding buffer. And pool into 50 ml Falcon tubes (try not to make subbles).Use 3.2 ml/cm 12-14,000 MW dialysis tubing. Heat tubing in 500 ml H2O in microwave. Not boiling. Rinse tubing in fresh H2O several times
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
for your particular application. Do not use an excess volume of Dynabeads Mouse T-Activator CD3/CD28, as excess Dynabeads per cell may inhibit expansion. Prior to re-stimulation, remove the used Dynabeads by transferring the cells to a suitable tube. Place
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