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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse, Monoclonal/IgG2b, kappa
- 形态:
液体
- 保存条件:
2-8℃
- 克隆性:
单克隆
- 适应物种:
Human; Mouse (predicted: Rat)
- 保质期:
一年
- 级别:
病理级
- 库存:
100
- 供应商:
ImmunoWay Biotechnology Company
- 宿主:
小鼠
- 应用范围:
IHC
- 规格:
3ml/6ml/10ml
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
pellets in 10 ml of 100 mM Tris buffer or Binding buffer. And pool into 50 ml Falcon tubes (try not to make subbles).Use 3.2 ml/cm 12-14,000 MW dialysis tubing. Heat tubing in 500 ml H2O in microwave. Not boiling. Rinse tubing in fresh H2O several times
Th17 Polarization of Mouse CD4 Cells
in complete medium and use your favorite method to isolate CD4 cells. Check out Biocompare.com to find useful kits. 2. Th17 Polarization of CD4 cells 1) Coat 60×15 mm of plastic petri dishes with anti-mouse CD3ε, clone 145-2C11(2&mu
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