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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
MR1
- 标记物:
FITC
- 规格:
100 ug
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文献和实验分钟收集细胞; 5. 将上一步离心得到的细胞沉淀,重悬至扩增培养基中,并进行计数。 1.3 细胞刺激(次日) 扩增培养基成分:RPMI1640 + 10% 血清 + 5ug/ml anti-mouse CD28(克隆号:37.51) + 双抗 1. 将细胞浓度调整至 1-2×10^6/ml(本次实验为 2×10^6/ml,该浓度偏大); 2. 从 4°C 冰箱取出前一天包被的板子,弃掉包被液,无菌PBS洗涤3遍; 3. 每孔加入 500μl 细胞悬液,放置于 37°C 含 5% CO2 细胞
CD8+, CD8, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand
represent the migratory and inflammatory DC subtypes and not the DC subtypes found in the steady state. By contrast a different culture method was described where mouse bone marrow is cultured with flt3 ligand for 9 days. Here, we describe this method
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
volume of Dynabeads taken from the vial (step 2). 2. Activation of Mouse T Cells 1) Start with 8 × 104 purified T cells in 100-200 µl medium in a 96-well tissue culture plate. 2) Add 2 µl Dynabeads Mouse T-Activator CD3/CD
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