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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
RM4-5
- 标记物:
FITC
- 规格:
500 μg
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文献和实验Th17 Polarization of Mouse CD4 Cells
in complete medium and use your favorite method to isolate CD4 cells. Check out Biocompare.com to find useful kits. 2. Th17 Polarization of CD4 cells 1) Coat 60×15 mm of plastic petri dishes with anti-mouse CD3ε, clone 145-2C11(2&mu
。 ④其他试剂和材料 l%叠氮化钠、离心机及离心管、烧杯(25m1)、搅拌器、无菌吸管及毛细滴管、烧杯(500m1)透析袋等。 (2)方法及步骤 ①取高效价的抗人球蛋白兔免疫血清,分离球蛋白,用盐水(0.15mol/LNaCl)及缓冲液(0.5mol/LNaHC03—Na2C03,pH9.0)稀释使每毫升内含蛋白质lOmg,缓冲液为总量的10%,降温至4℃。 ②加入异硫氰酸盐(FITC)荧光素[蛋白:荧光素=(50—80)mg‘lmg],在0~4~C下电磁搅拌12~
Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along the CD4/CD8 developmental pathway by a highly ordered process termed thymic selection ( 1 , 2 ). The maturation status of thymocytes is commonly assessed
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