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- BioGems
- 08011-25-500
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
EOS9.1
- 标记物：
SAFIRE Purified
- 规格：
500 ug
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文献和实验Electroporation is a method for introducing DNA into cells by using a high-voltage electric field. This method is very simple and easily manipulated. We describe here a method for the modification of tumor cells with the Fas/Apo-1 (CD95) antigen
and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
then recovered and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1% b-mercaptoethanol, 0.02% glusulase and 5ug/ml zymolyase. The suspension
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