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- 详细信息
- 文献和实验
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- 克隆性:
IM7
- 标记物:
SAFIRE Purified
- 规格:
1 mg
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文献和实验Phycoerythrin conjugation protocol
, about 2.5 mg/ml PE R-Phycoerythrin, purified (Molecular Probes P-801 in 60% ammonium sulfate) PBS Phosphate buffered saline without Ca2+ /Mg2+ MeOH Methanol
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
al. 2006b ). MATERIALS Reagents Anti-CD24 monoclonal antibody (mAb) (J11d clone) (for Protocol 3) Use either culture supernatant that contains anti-CD24 mAb or purified anti-CD24 mAb (see Step 55). BDPharmLyse (red blood cell lysing
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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