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- BioGems
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- 2025年07月12日
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- 克隆性：
2.00E+07
- 标记物：
APC
- 规格：
25 ug
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
solution and replace with mouse anti-biotin antibody diluted 1:100 in blocking solution for 40 minutes at 37C. We currently use DAKO antibody #M0743, 100-200m l per slide. (10) Wash with blocking solution for 10 minutes (11) Incubate with rabbit
40, then again with PBS/0.1 BSA. Mount solution (70 glycerol containing 2 n-propyl gallate and 0.25 ug/ml Hoechst) is placed on the cells. A coverslip is placed and sealed with nail polish. Slides are stored at -20oC until examination. Antibodies mouse
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
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