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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
145-2C11
- 标记物:
APC-Cy7
- 规格:
25 ug
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文献和实验Cyanine Dye Purification Protocol
Low salt buffer (LSB): 25mM triethylammonium acetate, pH 8.0 10% acetonitrile (this is 40-fold dilution of HSB + 10% acetonitrile) HSB, 800 mL To 500mL ddH2 O , add approx. 25-30 mL glacial acetic acid
Anti-factor Xa (Anti-Xa) Assay
The anti-factor Xa (anti-Xa) assay is a functional assay that facilitates the measurement of antithrombin (AT)-catalyzed inhibition of factor Xa by unfractionated heparin (UFH) and direct inhibition of factor Xa by low-molecular-weight
40, then again with PBS/0.1 BSA. Mount solution (70 glycerol containing 2 n-propyl gallate and 0.25 ug/ml Hoechst) is placed on the cells. A coverslip is placed and sealed with nail polish. Slides are stored at -20oC until examination. Antibodies mouse
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