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- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
1A8
- 标记物：
BG Violet 500
- 规格：
25 ug
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文献和实验LIVE/DEAD® Violet Viability/Vitality Kit
AM (Component A) to prepare a stock solution. Add 40 μL of this stock solution to 1.25 mL of buffer or medium to make a working solution of calcein violet AM. This working solution should be used within one day. 5. Add 1 μL aqua-fluorescent
and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
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