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- 2025年07月10日
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- 克隆性：
RM4-5
- 标记物：
BG Violet 500
- 规格：
25 ug
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文献和实验Th17 Polarization of Mouse CD4 Cells
in complete medium and use your favorite method to isolate CD4 cells. Check out Biocompare.com to find useful kits. 2. Th17 Polarization of CD4 cells 1) Coat 60×15 mm of plastic petri dishes with anti-mouse CD3ε, clone 145-2C11(2&mu
LIVE/DEAD® Violet Viability/Vitality Kit
AM (Component A) to prepare a stock solution. Add 40 μL of this stock solution to 1.25 mL of buffer or medium to make a working solution of calcein violet AM. This working solution should be used within one day. 5. Add 1 μL aqua-fluorescent
and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
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