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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse, Monoclonal/IgG1, Kappa
- 形态:
液体
- 保存条件:
-20
- 克隆性:
单克隆
- 适应物种:
Human; not react with Mouse; Rat
- 保质期:
一年
- 级别:
病理级
- 库存:
100
- 供应商:
ImmunoWay Biotechnology Company
- 宿主:
小鼠
- 应用范围:
WB;ELISA
- 靶点:
p27
- 规格:
40uL/100ul/200ul
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文献和实验Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
Mouse p27 PCR Using Gitschier Buffer
Mutant allele: Neo-1 primer (CCTTCTATCGCCTTCTTG), plus mgK-3 primer (TGGAACCCTGTGCCATCTCTAT) produce a 0.5kB PCR product. Wildtype allele: mgK-3 (above) plus mcK-5 (GAGCAGACGCCCAAGAAGC) produce ~1kB product. Stock Solutions
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
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