EZ Cap™ Cy5 EGFP mRNA (5-moUTP

Expression Profiling Using cDNA Microarrays

in a miniaturized gridded pattern, so that nanoliter amounts of tens of thousands cDNA samples are bound to a single 7.5 � 2.5 cm glass slide. Probes are generated from RNA samples of test and control tissues by incorporating Cyanine dyes (Cy™3 or Cy5) in reverse

细胞凋亡检测技术与方法

（bcl-2 和bcl-X）的调节物，它们的表达水平比例决定了细胞是凋亡还是存活。一般多采用Northern杂交和RT-PCR走胶对它们进行检测。随着近年来荧光定量PCR技术的发展，用定量PCR技术来检测基因表达水平无疑比之前者更快更准确。Intergen公司的Amplifluor™ Apoptosis Gene Systems就根据这一新技术原理，通过检测fas， bax-alpha 和 bcl-X （长的） 基因的 mRNA表达水平来进行细胞凋亡的检测。

细胞渗透蛋白（CPP）

or mRNA binding or by perturbing specific protein– protein interactions [Figure 1(c, d)]. Proteins linked to CPPs have shown an increase in membrane translocation and the effect of increased protein uptake has been shown in vivo and in vitro [22]. Examples