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- 文献和实验
- 技术资料
- 保存条件:
储存于-20 ° C
- 保质期:
1年
- 英文名:
Biotin-XX hydrazide
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
无
- 规格:
5mg
Biotin-XX hydrazide,Biotin-XX hydrazide,Biotin-XX hydrazide,Biotin-XX hydrazide
Biotin-XX hydrazide-Biotin-XX hydrazide
产品货号:APE-C4219-5
产品规格:5mg
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Biotin-XX hydrazide-Biotin-XX hydrazide储存于-20 ° C
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文献和实验Protein Carbonyl Determination Using Biotin Hydrazide
Protein oxidation is a recognized component of aging and a consequence of severe or prolonged exposure to reactive oxygen species (ROS). Direct attack of protein by ROS causes formation of protein-bound carbonyl groups (1 ). These carbonyl
by partial digestion of HA with ovine testicular hyaluronidase, and the oligosaccharides were labeled with biotin hydrazide and purified by a combination of aggrecan G1 domain and avidin affinity chromatography. Hyaladherins were prominently visualized
with 2,4-dinitrophenylhydrazine (DNPH) and detection of the protein-bound hydrazones using an enzyme-linked anti-2,4-dinitrophenyl antibody and (ii) derivatization with biotin-hydrazide and detection of the protein-bound acyl hydrazone with enzyme-linked avidin or streptavidin
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