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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
该EPI300 Competent Cell产品的保存方式具体查看产品。
- 保质期:
1年
- 英文名:
EPI300 Competent Cell
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
无
- 规格:
10X100ul
EPI300 Competent Cell,EPI300 Competent Cell,EPI300 Competent Cell,EPI300 Competent Cell
EPI300 Competent Cell-EPI300 Competent Cell
产品货号:APE-BC2002-1
产品规格:10X100ul
一种适合用于各种不稳定DNA或毒性克隆的感受态,可用于基因克隆、质粒转化和蓝白斑筛选等实验

Cell Biology|Competent Cells

EPI300 Competent Cell-EPI300 Competent Cell该EPI300 Competent Cell产品的保存方式具体查看产品。
点击:EPI300 Competent Cell-EPI300 Competent Cell查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多APExBIO公司特色小分子抑制剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验实验概要 Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate foreign DNA. On example of a competent cell is E. coli.
he cell suspension at 4,000 RPM in the Sorvall GSA rotor for 10 minutes. Step 5: Resuspend the bacteria pellet on ice in 1/20 volume of ice cold CaCl2 and then add an addional 9/20 volume of CaCl2. Keep this suspension on ice for at least 20 minutes
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
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