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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
该F-DH5α Competent Cell产品的保存方式具体查看产品。
- 保质期:
1年
- 英文名:
F-DH5α Competent Cell
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
无
- 规格:
10X100ul
F-DH5α Competent Cell,F-DH5α Competent Cell,F-DH5α Competent Cell,F-DH5α Competent Cell
F-DH5α Competent Cell-F-DH5α Competent Cell
产品货号:APE-BC2000-1
产品规格:10X100ul
一种可支持快速转化的DH5α感受态,其广泛用于基因克隆、质粒转化和蓝白斑筛选等分子生物学实验

Cell Biology|Competent Cells

F-DH5α Competent Cell-F-DH5α Competent Cell该F-DH5α Competent Cell产品的保存方式具体查看产品。
点击:F-DH5α Competent Cell-F-DH5α Competent Cell查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多APExBIO公司特色小分子抑制剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验实验概要 Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate foreign DNA. On example of a competent cell is E. coli.
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
he cell suspension at 4,000 RPM in the Sorvall GSA rotor for 10 minutes. Step 5: Resuspend the bacteria pellet on ice in 1/20 volume of ice cold CaCl2 and then add an addional 9/20 volume of CaCl2. Keep this suspension on ice for at least 20 minutes
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