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文献和实验, as well as (3) correcting for sample concentration-dependent inner-filter effects. The importance of the National Institute of Standards and Technology (NIST) traceable calibration and correction protocols is explained in light of valid intra- and interlaboratory studies
Determination of Molecular Size by Size‐Exclusion Chromatography (Gel Filtration)
calibration standards (see recipe ) Solution in mobile phase of protein whose size is to be determined
Size Measurement of Nanoparticles Using Atomic Force Microscopy
are provided. The nanoparticles cited are National Institute of Standards and Technology (NIST) Au nanoparticle Reference Materials RM 8011 (nominally 10 nm particles), RM 8012 (nominally 30 nm), and RM 8013 (nominally 60 nm).
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