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- 2025年07月08日
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文献和实验In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue
to perform with higher brightness and exposed to site-directed mutagenesis to generate proteins with emission spectra in the far-red region. The resulting Katushka was reported to be 7- to 10-fold brighter than other far-red fluorescent proteins, e.g., HcRed
Preparation of Fluorescent DNA Probe from mRNA: Yeast
DTT (0.1 M) 3.0 7.5 10 mM 50X dNTPs 0.6 1.25 500 uM; 200 uM dTTP Cy3- or Cy5 dUTP 3.0
Staining Methods for cell death Z. Xia 10/2/95
The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead cells 35 mm plates: to 2 ml medium or PBS, add 2 ul 2 mg/ml P
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