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文献和实验Phagocytosis Fluorescent labeling of yeast Resuspend 5 grams of yeast in 50 ml PBS in 100 ml flask Put flask for 30 minutes in boiling water bath while stirring. Wash 5 x with PBS
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dense products have been devised. A chromogenic substrate, 5-bromo-4-chloro-3-indolyl phosphate (X-Phos), which is very similar to X-gal, can be employed for detection and leads to production of a blue precipitate (Figure 1). In addition, the tetrazolium
Preparation of Para-magnetic beads from Promega cat#Z5482: a) suspend magnetic particles in bottle - transfer 200 ul (200 ug) of beads per sample of RNA to be purified to a microfuge tube or tubes. b) place microfuge tube
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