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- 百欧泰(biotyscience)
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- 2025年12月05日
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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
laser. Check the target slide for an ablation spot 1-10 um in diameter. Sequentially remove ND filters until an ablation spot is seen. Adjust the ablation spot to the center of the image with the rail mirror. You will want to add back a ND filter
Construction and Characterization of Adenovirus Vectors
an extinction coefficient of 1.1 x 1012 for wild-type Ad (Maizel et al. 1968 ), calculate the number of particles/milliliter: (OD260 )(dilution factor)(1.1 x 1012 ). A typical Ad vector preparation should have a ratio of particle to pfu of ~10
Creation and Use of Infectious Virus Vector
for this; polystyrene tubes DO NOT work!). 1 ug retroviral DNA encoding gene X 1 ug packaging plasmid (such as pCLEco, pCLampho, pUMVC, pHR’CVM8.2 deltaR etc.). If you are using a 3 plasmid system then:
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