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文献和实验Monitoring of Subvisible Particles in Therapeutic Proteins
, as outlined in the United States Pharmacopeia (USP) General Chapter or the equivalent Ph Eur 2.9.19 and the Japanese Pharmacopeia General Chapter 20. These methods were designed to protect patients against the risk of capillary occlusion though the infusion
Preparation of Fluorescent DNA Probe from mRNA: Yeast
DNA or RNA (10 mg/mL in TE) 1.0 uL 20X SSC 3.0 uL Millipore filter, 0.45 um 1 Millipore #UFC3OHVNB . *50X dNTPs stock
Preparation of Fluorescent DNA Probe from mRNA: Yeast
DTT (0.1 M) 3.0 7.5 10 mM 50X dNTPs 0.6 1.25 500 uM; 200 uM dTTP Cy3- or Cy5 dUTP 3.0
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