Fluorescent Labeled Magnetic B

Whole Body MRI and Fluorescent Microscopy for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following

Methods to monitor transplanted stem cells in vivo are of great importance for potential therapeutic applications. Of particular interest are methods allowing noninvasive detection of stem cells throughout the body. Magnetic resonance imaging

Basic Fluorescent in situ Hybridization (FISH)

[ Details ： Product No. L4390], 0.5-1.5 ng/µl labeled probe and 300 ng/ml Salmon Sperm DNA [ Details ： Product No. D7656] in 2x SSC.   Wash buffer: 20% formamide [ Details ： Product No. F7508] in 0.1x SSC.   Detection buffer

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA was constructed with a 5' biotin