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文献和实验Purification of DNA‐Binding Proteins Using Biotin/Streptavidin Affinity Systems
of DNA?either natural or formed from oligonucleotides?containing a high?affinity site for a DNA?binding protein provide a powerful tool for purification. The biotin/streptavidin purification system is based on the tight and essentially irreversible
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
communication) (1ul 2.65% Polystyrene 0.1 um in water) are added to the center of the pad followed by 3-5 worms oriented so they are lying on their left sides. A glass coverslip is applied and then Vaseline is used to seal the coverslip and prevent evaporation
8, CD14, CD19; 小鼠: CD4, CD8a, CD14, CD45R/B220, CD90.2 (Thy1.2), CD11b, Ly-6G. ë 利用Streptavidin连接的磁珠,只需选配biotin标记的所需单抗,就能分离更多种类细胞 新货聚焦: 现在,BD PMG又推出用于分离小鼠造血干细胞/祖细胞的新试剂mouse lineage panel。 其中包括5种biotin标记单抗
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