Recombinant SARS-COV-2 N (B.1.

高福院士团队 Lancet 发文：北京 COVID-19 病例分析表明，疫情爆发期间未出现新变种

导读 COVID-19 大流行已经持续了近 3 年，目前仍然是一个全球关注的热点话题。鉴于新冠病毒的广泛传播和不断演变，全球出现了众多变种，比如 alpha (B.1.1.7)、beta (B.1.351)、gamma (P.1)、delta (B.1.617.2)、omicron (B.1.1.529) 等。其中，Omicron 最早在南非被发现，增加的高传播力使其迅速成为全球范围内的优势毒株。 2022 年 12 月之前，中国采取了与其他国家不同的应对策略，从 2020 年的武汉封

Recombinant DNA Engineering, Or Cloning Genes In Plasmids

and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal

Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C

, and usually final protein yield between 0.1 and 2 mg/g cells is acceptable. Another concern is protein degradation. Especially with Pichia , protease activity during cell lysis and purification is always an issue. The importance of N-terminal degradation