Recombinant Mouse IgG1 Fc Pro

Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein That Provides a Cell Surface Matrix for Extended Culture and Efficient

and growth factors derived from mouse Engelbreth–Holm–Swarm �sarcoma cells. The complexity and lot-to-lot variation of Matrigel preparations has prompted the search for more defined substrates that can support pluripotent stem cell culture. A recombinant

Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation

cells. We present here (i) a detailed protocol for the production and purification of soluble recombinant NK cell receptors tagged with human IgG1-Fc (thus termed receptor-Fc chimera or receptor-Ig fusion protein) and (ii) a protocol for cell staining

Isotype-specific immunostaining with mouse primary antibodies on mouse tissue

  Staining mouse tissue with anti-mouse Ig (H&L chain) secondary reagents inevitabily causes staining of interstitial immunoglobulins, B cells, plasma cells and macrophages with Ig bound to Fc