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- 百欧泰(biotyscience)
- 北京
- BIOP2115
- 2025年07月02日
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文献和实验Methods to Detect Protein Glutathionylation
recombinant protein separated by nonreducing SDS‐PAGE (see protocol 1 ) Coomassie brilliant blue R‐250 staining solution (see recipe )
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
Strategies to Optimize Protein Expression in E. coli
., Cantor, E.J., Liao, W., Xu, M.Q., and Benner, J. 1998. Utilizing the C‐terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res. 26:5109‐5115
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