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文献和实验8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
Dynabeads® Co-Immunoprecipitation Kit
volume of antibody C1 (according to Table 1) to the washed beads and mix by vortexing or pipetting. Example: If you are coupling 5 mg Dynabeads and your required quantity of antibodies has a volume of 100 μl, you will need to add 150 μl of C1 (i.e. 250
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