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文献和实验I-a expression by glucocorticoids:the glucocorticoid receptor inhibits the DNa binding of the X box DNA binding protein.J Exp Med ,1993;177:691 10.Chao CC,et al.Activated microglia mediate nediate neuronal cell injury via a nitric oxide mechanism.J Immunol
Analysis �Protein A/G Bead Method. Cross-Linking, Sonication/Chromatin Shearing Efficiency. Reaction UHN Microarray Centre Chromatin IP (CHIP assay) - http://www.fhcrc.org/science/labs/breeden/Methods/chromatinIP.html ChIP assay protocol
Immunohistochemistry: Fluorescence Protocol 2.0
cytokine-specific antibodies (0.5-5 µg/mL) diluted in Wash Buffer-Saponin supplemented with 5% human AB serum. Wash the cells twice by centrifugation in Wash Buffer-Saponin (400 x g for 5 minutes). Incubate cells for 30 minutes at room
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