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文献和实验Th17 Polarization of Mouse CD4 Cells
Anti-mouse CD28, clone37.51[Details: LEAF™format, cat.#102112] Anti-mouse IL-4 [Details: LEAF™format, cat#504108] Anti-mouse IFN-γ [Details: LEAF™format, cat.#505812] Recombinant mouse IL-6(carrier-free)[Details: Biolengend, cat
High Throughput Production of Recombinant Human Proteins for Crystallography
, to be applied based on specific knowledge of protein families or when the core protocol is unsatisfactory. This scheme has been applied to more than 550 human proteins (>10,000 constructs) and has resulted in the deposition of 112 unique structures
Isolate or Deplete Human CD4+ T Cells - Whole Blood, Buffy Coat, MNC
/buffy coat to remove interfering soluble factors. （1）Dilute the whole blood or buffy coat in Buffer 1 (1 2). （2） Centrifuge at 600 x g for 10 min at 2-8°C. （3） Discard the plasma fraction/upper layer. Resuspend blood to the original
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