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文献和实验Steps for Standard PCR Reaction Design primers. In general, primers should have the following properties: Length of 18-24 bases 40-60% G/C content Start and end with 1-2 G/C pairs
Stewart Laboratory Standard Operating Procedure
BrdU Incorporation Protocol Day 1 Label cells with media containing100 μM BrdU (6 μL/mL of 5 mg/mL stock): Diploid Fibroblasts, 4-6 hours ES cells, 30 minutes 3T3 cells, 2.5 hours Trypsinize cells, wash from plate with PBS, spin down (400
Protein Particles for Pulmonary Drug Delivery
Pulmonary delivery of proteins requires particles for delivery to be in the aerodynamic size range 1–5 μm for deep lung deposition. However, the traditional particle size reduction technique of jet-milling normally used for inhalation
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