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文献和实验Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads
during the amplification reaction. The DNA can then be immobilized onto streptavidin-coated paramagnetic beads, simultaneously removing buffers, dNTPs, and unincorporated PCR primers. By alkali denaturation, the immobilized double-stranded DNA is converted into a single
by immunopurification of the CD19- or CD20-expressing cells with magnetic beads coated with an anti-CD19 or anti-CD20 antibody. The purified cells can be used for a wide range of biochemical analyses, including protein extraction for Western blot and immunoprecipitation
Cell Sorting Using Immunomagnetic Beads
Immunomagnetic beads are uniform, polymer particles coated with a polystyrene shell that provides both a smooth hydrophobic surface to facilitate physical absorption of molecules, such as antibodies, and surface hydroxyl groups that allow
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