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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. Centrifuge the Gelvatol solution at 12,000 rpm for 15 min. in 30 ml Corex tubes in Beckman J2-21 centrifuge at room temperature to remove undissolved particles. Pipette the supernatant into small screw cap bottles. Check pH of Gelvatol solution
Immunofluorescence Microscopy of tissue culture cells
Centrifuge 12,000 rpm, 15 min, to remove undissolved particles. The pH should be 6-7. Add 1.5g n-propylgallate with 30 ml Gelvatol to the supernate. Stir overnight. Store in small aliquots at -20 degrees C. METHOD:
Recovering DNA from agarose gels
a membrane with a small pore size, such as a Costar Spin-X microfuge tube fitted with a 0.22 um filter [12]. Alternatively, gel slices composed of low melting point (LMP) agarose can be placed in a microfuge at 70 degrees C until the gel has melted
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