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- 2025年07月12日
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. Centrifuge the Gelvatol solution at 12,000 rpm for 15 min. in 30 ml Corex tubes in Beckman J2-21 centrifuge at room temperature to remove undissolved particles. Pipette the supernatant into small screw cap bottles. Check pH of Gelvatol solution
Immunofluorescence Microscopy of tissue culture cells
Centrifuge 12,000 rpm, 15 min, to remove undissolved particles. The pH should be 6-7. Add 1.5g n-propylgallate with 30 ml Gelvatol to the supernate. Stir overnight. Store in small aliquots at -20 degrees C. METHOD:
A Method for Micro-Scale Isolation and Purification of Gangliosides
1-butanol, reagent grade Double distilled deionized H2 O (ddH2 O) Saline solution: 0.1% NaCl or 0.3% NaCl 15 or 50 ml glass conical Corning Centrifuge tubes with teflon- lined caps (#8142)
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