- 询价
- 百欧泰(biotyscience)
- 北京
- ABZW-13-70
- 2025年07月13日
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文献和实验Protein Syntheses in Cell Free Systems
Materials •Suspension culture of fibroblast cells (1 liter)•35 mM Tris-HCl,pH 7.4,140 mM NaCl (TBS buffer)•10 mM Tris-HCl,pH 7.5,10 mM KCl,and 1.5 mM magnesium acetate (TBS-M)•10X TBS-M200 mM Tris-HCl,pH 7.5,1200 mM KCl,50 mM magnesium acetateand 70
system. 3. SIFs and SACS Trisodium citrate was supplied by Spectrum. Silver nitrate and glucose were from Sigma-Aldrich. Microscope slides were coated by EMF Corp. (Ithaca, NY). A 52-nm thick layer of silver was deposited on the slide
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
antibody against surface marker of interest 0.1% paraformaldehyde in PBS 70% EtOH in glycerol (stored at 4°C) staining buffer (1% BSA in PBS with 0.005% Tween 20 and 0.1% sodium azide) propidium iodide (50 m g/ml solution with 100 U/ml DNase-free
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