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文献和实验SSR GEL and Silver Staining Protocol
with the quality and reproducibility. This would be an option for the silver staining work as well. The burst-pack's eliminate the c hemical weighing and mixing, deionizing, filtering and degassing steps. IV. GEL POURING: 1. Immediately prior to pouring, add 500
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr(图)
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
system. 3. SIFs and SACS Trisodium citrate was supplied by Spectrum. Silver nitrate and glucose were from Sigma-Aldrich. Microscope slides were coated by EMF Corp. (Ithaca, NY). A 52-nm thick layer of silver was deposited on the slide
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