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- 帛科
- BK-KT11286
- 国产
- 2025年07月10日
- WB
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海帛科
- 库存：
40
- 克隆性：
单克隆
- 抗原来源：
Mouse
- 抗体英文名：
Anti-ESRP1/2 Antibody (Clone#23A7.C9)
- 抗体名：
Anti-ESRP1/2 Antibody (Clone#23A7.C9)
- 标记物：
mouse
- 免疫原：
Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
- 亚型：
IgG1
- 形态：
Liquid
- 应用范围：
WB
- 浓度：
1.0 mg/mL by UV absorbance at 280 nm
- 规格：
100μg
| 指标别称：ESRP1; RBM35A; RNA binding motif protein 35A; RNA binding protein 35A; ESRP2; RBM35B; RNA binding motif protein 35B; RNA binding protein 35B 产品类型：Monoclonal 检验物种：mouse 产品类型：Monoclonalmouse 应用范围：WB 基因名称：ESRP1/ESRP2 克隆号：23A7.C9 抗体来源：Mouse 抗体亚型：IgG1 免疫原：Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein. 成分：0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide 纯化方式：This antibody was purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped. 浓度：1.0 mg/mL by UV absorbance at 280 nm 产品形态：Liquid 保存条件：Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. 应用释义：WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法; 基因名全称：Epithelial splicing regulatory protein 1 |
| 产品名称 | Anti-ESRP1/2 Antibody (Clone#23A7.C9) | 规格 | 100μg |
| 产品类型 | Monoclonal | 检验物种 | mouse |
| 应用范围 | WB | 基因名称 | ESRP1/ESRP2 |
| 抗体来源 | Mouse | 抗体亚型 | IgG1 |
单克隆跟多克隆的区别：
· 多克隆抗体多克隆抗体是动物体内生成多种不同抗体B细胞的活化结果。它们被视为抗体混合物，可与抗原表面的多个表位反应多克隆抗体抗原识别灵活性更高，亲和力更强，兼容更广泛的应用。但是同时也可能产生非特异性结合带来的高背景和批次间的不稳定性。
· 单克隆抗体单克隆抗体通过杂交瘤技术生产，每个B细胞产生针对特定抗原表位的特异性抗体，同一克隆的抗体产生完全相同的抗体拷贝。因此单克隆抗体具有高特异性、批间一致性和重复性等特点。然而单克隆抗体可能会出现识别抗原丢失或隐蔽，实验应用受限等问题，杂交瘤细胞系漂变也会影响单抗的表达。
| IL22RA2 Antibody Blocking Peptide |
| Human Cyclic guanosine monophosphate, cGMP Elisa Kit |
| Gibberellins/BSA |
| CD55 Antibody Blocking Peptide |
| Human Leukotriene B4, LT-B4 Elisa Kit |
| FGF3 Antibody Blocking Peptide |
| Human 25-Dihydroxy vitamin D3, HVD3 Elisa Kit |
| DEFB134 Antibody Blocking Peptide |
| Human Bone morphogenetic protein 7, BMP-7 Elisa Kit |
| RNF8 Antibody Blocking Peptide |
| Human skeletal muscle Actinin-α, sm Actinin-α Elisa Kit |
| SIK1 Antibody Blocking Peptide |
| Human Endothelial lipase, EL Elisa Kit |
| Phospho-Bcr (Tyr177) Antibody Blocking Peptide |
| Human lipoprotein-associated phospholipase A2, Lp-PL-A2 Elisa Kit |
| Human intestinal fatty acid binding protein, iFABP Elisa Kit |
| C6orf35 Antibody Blocking Peptide |
| TRAF3IP3 Antibody Blocking Peptide |
| Anti-ESRP1/2 Antibody (Clone#23A7.C9) |
| Human adiponectin, ADP Elisa Kit |
| GPATCH11 Antibody Blocking Peptide |
| Human Myeloid Progenitor Inhibitory Factor 1, MPIF-1 Elisa Kit |
| TLR5 Antibody Blocking Peptide |
| Human oxidized lowdensity lipoprotein, OxLDL Elisa Kit |
| Rat nitric oxide, NO Elisa Kit |
| PAX1 Antibody Blocking Peptide |
| human Histamine, HIS Elisa Kit |
| IRS P53 Antibody Blocking Peptide |
| phospho-PLB (Thr17) Antibody Blocking Peptide |
| human Resistin Elisa Kit |
1 免疫动物选择：
根据需要产生目标抗原的免疫反应，选择合适的免疫动物。例如，小鼠是最常用的免疫动物之一，因其易于操作且在免疫应答方面较为强大。
2 抗原免疫
将目标抗原注射到免疫动物体内，通常需要多次免疫以激发免疫反应。可以选择不同的免疫路线，如皮下、腹腔、静脉等。
3 细胞融合
从免疫动物获得脾细胞或淋巴细胞，与骨髓瘤细胞或其他癌细胞（如SP2/0、NS0等）融合，形成杂交瘤细胞。融合细胞通常使用聚乙烯醇（PEG）或电穿孔等方法促进细胞融合。
4 杂交瘤细胞筛选
对融合细胞进行筛选，一般使用HAT选择性培养基，通过对非杂交瘤细胞和单克隆杂交瘤细胞的抗代选择，筛选出产生目标抗体的单克隆杂交瘤细胞。
5 单克隆抗体生产
从筛选出的单克隆杂交瘤细胞中获取单一细胞系，并进行大规模培养和产生抗体。一般可采用体外或体内方式生产单克隆抗体。
6 抗体纯化
通过不同的方法对产生的单克隆抗体进行纯化，去除杂质，例如使用蛋白A/G层析、蛋白L层析、蛋白G层析等。
7 鉴定和验证
对纯化后的单克隆抗体进行鉴定和验证，例如使用酶联免疫吸附实验（ELISA）、免疫印迹（Western blotting）等方法，确定其免疫特异性和活性。
8 应用
将制备好的单克隆抗体应用于具体的研究、诊断或治疗等领域，例如免疫组化、流式细胞术、免疫疫治疗等。
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文献和实验step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
below. Blocking step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
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