Nonspecific endonuclease cleaving single and double stranded DNA and RNA.
Buffer
50 mM Tris, 500 mM NaCl, 5 mM MgClz,50%(vlv) Glycerol, pH7.4.
QC Testing
Test
Standard
Protein quantification (UV)
0.2-0.3 mg/mL
Purity (SDS-PAGE)
≥ 95%
Enzyme activity*
≥3×106 U/mg
Endotoxin level
≤10 EU/mg
*Unit definition:One unit (U) is defined as an increase in absorbance at 260 nm of 1 Absorbance Unit in 30 minutes at 37℃, using overdose DNA substrate in a buffer with 50 mM Tris-HCl (pH 8.0, 25℃), 5 mM MgCl2, and 500 mM NaCl.
Working conditions
Working ranges
Optimal ranges
Temperature (℃)
4- 50
25- 45
pH
7.0 - 10.0
7.5-9.5
NaCl (mM)
50 - 900
350 - 600
Mg2+(mM)
1- 60
1-10
Note: The working range is defined as above 10% activity and optimal range as above 80% activity.
SDS-PAGE
Usage Guide
Storage
Store it under sterile conditions at -20°℃ upon receiving for at least 12 months.Recommend to aliquot the protein into smaller quantities for optimal storage.Avoid repeated freeze-thaw cycles.
Shipping
This product is supplied and shipped with blue ice.