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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 形态:
液体
- 保存条件:
在-20℃下储存有效期为12个月。 避免冻融循环。
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Mouse
- 保质期:
12个月
- 目录编号:
E-AB-70368
- 级别:
科研级别
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Rabbit
- 应用范围:
IHC
- 抗体英文名:
SIRT1 Polyclonal Antibody
- 抗体名:
SIRT1 多克隆抗体_货号:E-AB-70368_多克隆抗体
- 规格:
200μL/120μL/60μL
| 规格: | 200μL | 产品价格: | ¥3300.0 |
|---|---|---|---|
| 规格: | 120μL | 产品价格: | ¥2220.0 |
| 规格: | 60μL | 产品价格: | ¥1380.0 |

SIRT1 多克隆抗体产品名称:SIRT1 多克隆抗体, 货号:E-AB-70368
| 已验证样本 | 免疫组织化学(IHC)已验证样本:Mouse testis,Mouse brain,Mouse heart |
| 稀释 | IHC 1:200-1:600 |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 免疫原 | Recombinant protein corresponding to Mouse SIRT1 |
| Abbre | SIRT1 |
| 同义词 | 75SirT1;hSIR2;hSIRT1;HST2;S. cerevisiae;homolog of;NAD dependent deacetylase sirtuin 1;NAD dependent protein deacetylase sirtuin 1;OTTHUMP00000198111;OTTHUMP00000198112;Regulatory protein SIR2 homolog 1;SIR1;SIR2;SIR2 like 1;SIR2 like protein 1;SIR2;S.cerevisiae;homolog-like 1;SIR2-like protein 1;SIR2ALPHA;SIR2L1;Sirt1;SirtT1 75 kDa fragment;Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae);Sirtuin 1;Sirtuin type 1抗体 |
| Swissprot | Q923E4 |
| 细胞定位 | Cytoplasm. Mitochondrion and Nucleus, PML body. Cytoplasm. Nucleus. Recruited to the nuclear bodies via its interaction with PML (PubMed:12006491). Colocalized with APEX1 in the nucleus (PubMed:19934257). May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane (PubMed:15469825). Shuttles between nucleus and cytoplasm (By similarity). Colocalizes in the nucleus with XBP1 isoform 2 (PubMed:20955178). |
| 浓度 | 1.2 mg/mL |
| 储存方法 | 在-20℃下储存有效期为12个月。 避免冻融循环。 |
| 缓冲 | PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4 |
| 纯化方法 | Affinity purification |
| 研究领域 | Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Metabolism; Microbiology; Tags and Cell Markers |
| Conjugation | Unconjugated |
| 产品描述: | SIRT1,also named as SIR2L1,contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. It regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signalling events relevant to cardioprotection,including: activation of endothelial nitric oxide synthase,insulin receptor signalling,and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO,Hif-2a,and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53,specifically at Lysine 382. |

SIRT1 多克隆抗体labscience®拥有先进的单抗,多抗和流式抗体开发技术平台,提供8500+高品质 抗体, 适用于胞内和胞外蛋白的鉴定、定量和定位研究。抗体已在多种应用中得到验证,包含 流式细胞术(Flow Cytometry)、 免疫印迹(WB)、 免疫组化(IHC/ICC)、 免疫(共)沉淀(IP/Co-IP)、 免疫荧光(IF) 等实验。抗体产品涉及多种生命科学研究领域,包括肿瘤、免疫、心血管、细胞生物学、神经科学、表观遗传学等。Elabscience®始终致力于提高抗体的质量和种类,以保障并加快您的研究进程
在-20℃下储存有效期为12个月。 避免冻融循环。SIRT1 多克隆抗体产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验Methods to Investigate the Role of SIRT1 in Endothelial Senescence
Sirtuins are a family of proteins with NAD+ -dependent deacetylase or mono-ADP-ribosyltransferase activity. SIRT1, the mammalian ortholog most closely related to Sir2 (the first gene of this family discovered in yeast), exhibits anti
SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome
, in combination with affinity enrichment of acetyllysine peptides and mass spectrometry, to study the dynamic changes of the Lysine acetylome in response to Sirt1. The method should be applicable to quantify changes to other post translational modifications
实验原理 免疫电泳又称为γ球蛋白电泳或免疫球蛋白电泳技术,这是一种能够判断血液中三种免疫球蛋白IgM,IgG,IgA含量水平的实验方法。在这项实验技术中,首先利用蛋白质的分子量和所带电荷的比值运用水平琼脂糖凝胶电泳技术将蛋白质进行分离,然后将特异性的抗体引入到与电泳方向平行的凹槽中,在抗原和抗体的扩散过程中,抗原和抗体适当比例的结合会导致沉淀的产生。0.85%盐不仅可以终止扩散过程也可用于洗去未结合的蛋白,抗原和抗体结合所形成
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