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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
TNF alpha Polyclonal Antibody_货号:E-AB-33121_多克隆抗体
- 抗体英文名:
TNF alpha Polyclonal Antibody
- 靶点:
TNF-α
- 浓度:
5 μL/Test
- 应用范围:
WB;IHC-p;IF;ELISA
- 宿主:
Rabbit
- 适应物种:
Human;Mouse;Rat
- 保质期:
12个月
- 级别:
科研
- 库存:
99
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
- 形态:
液体
- 亚型:
IgG
- 规格:
200μL/120μL/60μL/20μL
| 规格: | 200μL | 产品价格: | ¥2365.0 |
|---|---|---|---|
| 规格: | 120μL | 产品价格: | ¥1640.0 |
| 规格: | 60μL | 产品价格: | ¥1010.0 |
| 规格: | 20μL | 产品价格: | ¥420.0 |
货号:E-AB-33121
| Verified Samples |
Verified Samples in WB:Jurkat
Verified Samples in IF:Human stomach |
| Dilution |
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000
|
| Clonality |
Polyclonal
|
| Isotype |
IgG
|
| Immunogen |
Synthesized peptide derived from the Internal region of human TNF-α
|
| Abbre |
TNF-α
|
| Synonyms |
APC1;APC1 protein;Cachectin;DIF;Differentiation inducing factor;Macrophage cytotoxic factor;Tnf;TNF superfamily;member 2;TNF;macrophage derived;TNF;monocyte derived;TNF-a;TNF-alpha;TNFA;TNFA;TNFSF2;Tumor necrosis factor alpha;Tumor necrosis factor;Tumor necrosis factor ligand superfamily member 2;Tumor Necrosis Factor;Membrane Form;Tumor necrosis factor;soluble form抗体
|
| Swissprot |
P01375
|
| Calculated MW |
26kDa
|
| Observed MW |
16kDa
|
| Cellular Localization |
Secreted and Cell membrane.
|
| Concentration |
1 mg/mL
|
| Storage |
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
|
| Buffer |
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
|
| Purification Method |
Affinity purification
|
| Research Areas |
Cancer; Cardiovascular; Metabolism; Immunology; Signal Transduction
|
| Conjugation |
Unconjugated
|
| Background |
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
|
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文献和实验Effect of fullerenol C60 on lung and renal tissue in lower extremity ischemia?reperfusion injury in sevoflurane?treated rats
IF:3.4
Journal:Molecular Medicine Reports
DOI:10.3892/mmr.2024.13178
【求助】有什么办法可以阻滞LPS刺激的TNF-alpha分泌?
sonny_zan 我实验的关键步骤,现在就是不知道有什么办法可以阻滞LPS刺激后PBMC分泌TNF-alpha,考虑过用p38 MAPK抑制剂SB203580,后来查到一篇文献说SB203580对LPS刺激后p38 MAPK的活性影响甚微或无。 请各位大侠给点意见啦!我快急死了~~~ xiongran 拿抗体把PBMC表面的TLR4封闭掉试试看! pureclear 可以考虑
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
Purification of TNF Binding Proteins
receptors. The purified proteins may be used in biological experiments or for the generation of specific research tools such as polyclonal or monoclonal antibodies.
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