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文献和实验Plant DNA methylation analysis by bisulfite genomic sequencing
and Wassenegger (2000). Back to top Procedure Preparation of Plant Genomic DNA Prepare plant genomic DNA using a Qiagen DNeasy Plant Maxi or Mini kit (see comment 1). Prior to the bisulfite-treatment, the plant genomic DNA is cleaved
Whole genome amplification protocol for ChIP-chip
to a genomic tiling array. Therefore, we have adapted the standard protocol for whole genome amplification using the Sigma GenomePlex WGA kit to amplify our ChIP sample (O'Geen et al., 2006). Using Oct4 ChIP-chip assays as an example, we have compared
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