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- AE90343Ca
- 2025年07月08日
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文献和实验reporter plasmids (TCF reporter plasmid kit; Upstate) is needed. B Pharmacological inhibition ofGSK-3 activity. MiaPaCa2 pancreatic cancer cells do not express E-cadherin and there is free (free from binding to Ecadherin)cytosolic β-catenin pool
such as Stbl3 (Life Technologies,Carlsbad, CA, USA) or other recombination-defi cient strain isrecommended to avoid recombination of repetitive elementsin the plasmids. 12. Luria Broth (LB) media. 13. Ampicillin. 14. Spin miniprep kit. 15. Plasmid
). Recently, Ambion introduced a kit (the Silencer™ siRNA Construction Kit) to produce siRNAs by in vitro transcription, which is a less expensive alternative to chemical synthesis, particularly when multiple different siRNAs need to be synthesized
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