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文献和实验Hormones as Biomarkers: Practical Guide to Utilizing Luminex Technologies for Biomarker Research
the principle of a sandwich ELISA with the fluorescent-bead-based technology allowing individual and multiplex analysis of up to 100 different analytes in a single microtiter well. Serum assay described in the methods section is performed in 96-well microplate
such as Stbl3 (Life Technologies,Carlsbad, CA, USA) or other recombination-defi cient strain isrecommended to avoid recombination of repetitive elementsin the plasmids. 12. Luria Broth (LB) media. 13. Ampicillin. 14. Spin miniprep kit. 15. Plasmid
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