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文献和实验Metallomics Using Inductively Coupled Plasma Mass Spectrometry
. Sci. U.S.A. 104:20641‐20642. Coligan, J., Dunn, B.M., Speicher, D.W., and Wingfield, P.T. 2012. Current Protocols in Protein Science. John Wiley & Sons, Hoboken, N.J
秀丽线虫RNA提取--C. elegans RNA prep
. 5. Pour the mixture into a 28 ml polycarbonate Oak Ridge tube. Spin in the 70Ti ultracentrifuge rotor for 20 minutes at 30K RPM at 20° to pellet debris (and any metallic stuff from the polytron). Pour off the supernatant into a clean 50 ml
metal? chunks; don't worry, you can spin these out.) 4. Add sarcosyl to 0.5 % (using the RNase free 20% solution) and mix. 5. Pour the mixture into a 28 ml polycarbonate Oak Ridge tube. Spin in the 70Ti ultracentrifuge rotor for 20 minutes at 30
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