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- Beckman Coulter贝克曼库尔特
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- 2025年07月08日
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文献和实验Synthesis of Peptide‐Oligonucleotide Conjugates by Diels‐Alder Cycloaddition in Water
on a 2‐Chlorotrityl Chloride Resin Basic Protocol 4: Preparation of Peptide‐Oligonucleotide Conjugates by Diels‐Alder Cycloaddition in Water Alternate Protocol 2: Preparation of Cysteine‐Containing Peptide
Extraction of genomic DNA from whole blood
pellet by vortexing vigorously for 30-60 seconds. Then add 50 µl of Proteinase K solution (20mg/ml). The Proteinase K solution should be made fresh and refrigerated prior to use. 4.Leave to incubate for two hours at 55℃ in a water bath. Remove samples
gel electrophoresis. Protocol 1. Prepare an early log phase culture of JM101 by inoculating an Ehrlenmeyer flask containing 50 ml of 2xTY with a glycerol stock of JM101 and pre-incubating for 1 hour in a 37degC water bath, with no shaking
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