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文献和实验Synthesis of Peptide‐Oligonucleotide Conjugates by Diels‐Alder Cycloaddition in Water
on a 2‐Chlorotrityl Chloride Resin Basic Protocol 4: Preparation of Peptide‐Oligonucleotide Conjugates by Diels‐Alder Cycloaddition in Water Alternate Protocol 2: Preparation of Cysteine‐Containing Peptide
Extraction of genomic DNA from whole blood
pellet by vortexing vigorously for 30-60 seconds. Then add 50 µl of Proteinase K solution (20mg/ml). The Proteinase K solution should be made fresh and refrigerated prior to use. 4.Leave to incubate for two hours at 55℃ in a water bath. Remove samples
Extraction of genomic DNA from whole blood
and refrigerated prior to use. 4.Leave to incubate for two hours at 55℃ in a water bath.Remove samples and leave t℃ool to room temperature (or leave for 2-3 minutes on ice).Add 4 ml of 5.3 M NaCl solution.Vortex gently for 15 seconds. 5.Spin at 4500 rpm for 15-20
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