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- Beckman Coulter贝克曼库尔特
- 美国
- 2083049-11
- 2025年07月14日
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文献和实验E-Z 96 Plant DNA Protocol-Vacuum Manifold Processing
at 4,000 x g for 10 minutes to dry the plate. 9. Assemble the manifold by placing a new 300 ul collection plate (supplied) inside the vacuum manifold. If an Omega VAC-03 is used, one 800 ul plate should be place under the 300 ul plate to give proper
E-Z 96® M13 Isolation Vacuum Manifold Protocol
实验试剂 1. Sterile deionized water (or TE buffer) 2. Absolute (96%-100%) ethanol 实验设备 1. Microcentrifuge capable of at least 10,000 x g 2. Sterile 15 mL centrifuge tubes 3. Sterile
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
is complete, about 5-20 minutes. 8. Remove lysate filtrate collection block from manifold. Add 2ul of glycogen type II (Sigma Inc.) at 1ug/ul and ice-cold isopropanol (0.7x volume of filtrate) to each well. Seal with plastic sealing tape and mix. Incubate
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