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文献和实验HIGH RESOLUTION GENETIC FOOTPRINTING
column can be loaded many times in order to concentrate the sample. If the concerted integration PCR product has a small amount of smearing, the PCR product should be gel purified using the Qiaquick gel extraction kit as described
A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform
of the target DNA from an origin vector and its subsequent integration into the destination vector. TPCR can be applied for incorporation of DNA fragments into any desired position within a circular plasmid without the need for purification of the intermediate
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
. Used with permission). STA-PCR and SplinkBlunt-PCR can consistently recovermore insertion sites of BXH-2 MuLV with high specificity (foran example, see Fig. 2). The high efficiency of SplinkTA-PCRmay be attributed to the integration
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