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- 2025年07月12日
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文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
over a piece of black cardboard, using a P-200 Gilson pick colony bringing with it the smallest possible volume of PBS. Pick 6 colonies in succession into a 96 well plate containing 20ul TRYPSIN/EDTA. Check using microscope that there are single colonies
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
5 2 x 106 EP + DNA G418 % total integrants ie random + H/R's 6 2 x 106 EP + DNA G418* Same as Plate 5 unless 7 2 x 106
Detection and Measurement of Radioactivity
can dislodge electrons from the atoms it strikes, producing pairs of ions. Particles are allowed to pass between parallel plates, one with a positive charge and one with a negative charge. As ionization takes place the ions each move to the plate
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