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文献和实验Lipoprotein Isolation - First week
the dissolved portion into a 50 ml measuring cylinder and add more (2-3 ml) PBS to the undissolved KBr and stir until dissolved. Pool these solutions and make up to 20 ml with PBS and stir further in the measuring cylinder. 9. Transfer solution into a 30
Lipoprotein Isolation - First week
off the dead animals at a later date. 6. Make up the hemolymph solution to ~15 ml with PBS. 7. Add 8.9 g of KBr crystals and stir gently using a clean stir bar. 8. Transfer the dissolved portion into a 50 ml measuring cylinder and add
in the past - Sigma #N-4388; so we now use powdered media from Gibco #21700-075 or liquid media from Gibco #11765-054. Neither of these have Hepes buffering) 1) Start by rinsing a graduated cylinder three times ddH2 O. 2) Then fill the cylinder to 600-ml
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