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文献和实验-20 in 50 mM Na-phosphate buffer, pH 7.3. Goat-anti-mouse antibodies labeled with AlexaFluor-488 were from Invitrogen, Inc. (dye/protein ratio 7). Microscope glass slides, 3 × 1 in., and 1 mm thick, were from VWR. Water was purified by Milli-Q
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
1 High-Energy Nd:YAG Laser Mirror, 25.4 mm Diameter, 45°, 532 nm Newport 10Q20HE.2 4 SUPREMA Optical Mount, 1.0 inch diameter
- - - - - - 5μM 5μM 0.5μM Nicotinamide - - - - - - 10mM 10mM - Forskolin - - - - - - 10μM 10μM - R-Spondin - - - - - - - 1μg/ml - CX83 FGF basic - 100ng/ml 5 ng/ml
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