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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
°C 5. Sterile serological pipettes (5-mL, 10-mL) 6. Centrifuge 7. 15-mL centrifuge tubes 8. 60-mm and 100-mm tissue culture-treated dishes 9. 6-well tissue culture-treated plates 10. 25-gauge 1½-inch needle
Buffer P1 50 mM Tris-HCl pH 8.0 10 mM EDTA 100 μg/ml RNaseA The buffer and RNaseA can also be ordered from Qiagen separately (catalog numbers 19051 and 19101). Buffer P2 200 mM NaOH 1% SDS Buffer P3
in liquid nitrogen using a mortar and pestle. b. Transfer between 5 and 100 mg of ground seed material into a pre-weighed 1.5 ml microcentrifuge tube. Record the mass of the transferred seed material. c. Pipette 4 ul of nuclease-free
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