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- Beckman Coulter贝克曼库尔特
- 美国
- 998911C
- 2025年07月10日
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Protocol to Count Cell Number of Preimplantation Embryos
the cells for 10 minutes. 3. Wash embryos two times in 100 μ l drops of PBS-PVP transferring the embryos drop to drop. Mounting Embryos to Slides 1. Prepare clean microscope slides by dipping in a 1:10 poly-l-lysine solution (Sigma P8920
KOH. Dissolve the paraformaldehyde by carefully heating the solution on a stirring hot plate in a fume hood. Once the solution cools down, filter through a 0.45 micron filter and store at 4°C until ready for use. Scale up the recipe if a larger
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